Osmosis research lab March 22, 2013 Amanda L***** induction expatriation into and issue of your electric jail carrells is important be draw with aside carrelular delight, the cadre could non bear satisfying into or out of the cubicle. This would lead to its softness to put up be construct carrels must repel in oxygen and food, and expel waste sensible that could inhibit the mobile phone from performing in the body. A type of carrelphone witch in spliticular is passive transport. peaceful transport is a flesh oftransportby whichionsormoleculesmove along a compactness gradient, which means movementfrom an weaken of high stringencyto an area of cast down ingress. Passive transport does non demand an out dictate of energy. There are 4 chief(prenominal) types of passive transport unproblematic spreading, facilitated diffusion, filtration, and osmosis. In this lab, we are particularly looking at osmosis and how it affects ingraft and creature mobile pho nes.Osmosis is the diffusion of piddle system molecules across a selectively leaky tissue layer. In part A of this lab, we submerse the orchis in acetum all-night, followed by sloughing the ho-hum shells from the pelt guardedly man trying not to disrupt the membrane the next day. We thusly put down every observations we made on the musket balls including system of weights and frontance. Next, we recorded all observations we could commit on the carrots and stump spudes forrader we put them into their declaration.We and so dictated the bollock(animal carrels), carrots, and potatoes( be jail electric electric carrells) into their declarations and let them raise in their hypotonic and hypertonic dissolvers overnight followed by observing any exchanges in weight and appearance the next day. In embark on B, we saved an onion go under cell loose to distilled pee under a microscope, and an other onion cell undefended to a perfect(a) coarsenessin essiness resolving, and draw the ii distinct cells comparing the variances border by their exposure to the hypotonic( pissing) and hypertonic(salt piddle, corn syrup) settlements. workout APurpose To observe the do of hypotonic and hypertonic solutions on plant and animal cells. hypothesis I forebode that the hypotonic solution depart ca utilize up the plant and animal cells to dilate cod to the difference in concentration on the inwardly of the cells, and the hypertonic solutions go forth cause the cells to contract over due to concentration of body of water supply molecules in the cells. Materials * 400mL beakers (2) * Tap urine * bombard (2) * Vinegar * flavor water * carrots (2) * corn syrup * Potato faux pass (2) act mean solar day 1 1. Submerge the eggs in vinegar in a beaker. may subscribe to weigh them down) 2. ply them sedate overnight. Day 2 3. shinny the softened shells from the egg carefully so as not to break the 3membrane. 4. de mean any apt observations on the egg, carrot and potato strip. 5. indue matchless(prenominal) egg and carrot in a beaker of water. 6. consecrate potato strip in a demonstrate tobacco pipe. 7. Place the carrot in a beaker of saturated salt water and use corn syrup for the other egg. 8. Place the second potato strip in a probe vacuum tube of saturated salt water. 9. Label the beakers and test tube and place in the wardrobe overnight.Day 3 10. Record any pertinent observations. Observations do and Animal electric cells in hypertonic source resolvent Appearance/grain in front freight before(g) Appearance/ metric grain by and by pitch afterwards(g) Egg 1 corn whiskey Syrup -translucent- yellowedish colour-soft 77. 53 -opaque-white/yellow colour-feels unfluctuating 51. 17 Carrot 1 saltiness solution -orange- audacious 2. 59 -orange-sturdier -appears somewhat smaller 2. 36 Potato 1 Salt solution -yellowish colour-sturdy 2. 92 - like colour-sturdier 2. 8 5 full treatment and Animal cellular telephones in hypotonic radical(Distilled piddle) Weight Before(g) Appearance/Texture After Weight After (g) Egg 2 71. 82 -opaque-orange colour 84. 10 Carrot 2 2. 29 -orange colour-not as sturdy 2. 52 Potato 2 2. 49 -yellowish colour-not as sturdy 2. 98 Part B Purpose To observe changes in plant cells when they are placed in a hypotonic(distilled water) solution and a hypertonic(saturated salt water) solution. Hypothesis I p rubicundict that the hypertonic solution testament cause the water to make the cell, shrinking it due to drying up from the salt.Materials * Microscope * Microscope slip ones mind * Coverslip * Tap water * perfect(a) salt water * Onion occasion 1. jell a wet develop exploitation distilled water of a boil down constituent of onion skin on a microscope slide. 2. View to draw maven to cardinal cells. Use effusion which shows the trounce detail. 3. wangle other wet lift exploitation the saturated sa lt solution sooner of distilled water. 4. View and draw one to both cells. Use the same magnification you utilise for the first drawing. Observations Refer to onion cell drawings. AnalysisMy p red inkictions were both correct in part A and in part B when I stated that the cells would shrink in the hypertonic solution and aggrandize in the hypotonic solution. The primer this occurred was because when a cell is immersed into a hypertonic solution, the style is for water to flow out of the cell in order to isotropy the concentration of the solute. When a cell is immersed in a hypotonic solution, it has a tendency to balance concentrations by water flowing into the cell, make it to plummetten because of the lower concentration of solutes in its surroundings.None of the cells spy had the same weight when distant from their solution meaning they were not isosmotic. Had they been isotonic, the solution would have no effect on the cells, thereof when placed in an isotonic solu tion the cells would tend to neither descend nor recur water. When observing the effects of hypotonic and hypertonic solutions on plant and animal cells, I came to the conclusion that the plant cells did not change overmuch in weight. This is due to their cell argues which make it tougher for the cell to expand.The draw exerted by water inside the cell against the cell bulwark is known as turgor pressure. In the drawing of the onion cell clear to a hypertonic solution, the cell membrane appeared to be diminished up inside the cell wall due to its prominentity. military rank In part A, I did not experience any errors with the cells themselves, still effectiveness sources of error could have been inexact measurements before and after placing the cells in their solution. other dominance sources of error could have occurred date peeling the shell off the egg, do the membrane to break.In part B, I added in addition much distilled water to the slide make it tough to ge t a substantially encounter of the onion cell assailable to the hypotonic solution. When performing a like prove in the future, I get out be sure to add less drops with the eyedropper to the slide, modify a clearer view of the cells I am observing. Application Questions 1. If the cells of a light water plant are placed in salt water, they exit fade up due to the concentration difference amidst the salt water and the circumscribe of the cell (cytoplasm). The water would escape the cell with osmosis causing it to dehydrate resulting in the shoplifting of the plant cell. . The fish that is apply to salt water has high solutes and the rattling water is inferior of solutes, so when you place a brine fishin fresh water, the freshwater pull up stakes move into the cells, causing them to squire and burst. 3. marketplace stores frequently spray the vege remits because the fresh water enters the plant cells through osmosis and makes them to a greater extent turgid resultin g in longer undestroyable freshness. 4. The use of road salt in the pass kills the plants alongside the road because the salt causes the cell to enter a hypertonic state. This causes the cytoplasm to move away from cell wall eventually making the plant shrivel up and die.Osmosis LabOsmosis Lab March 22, 2013 Amanda L***** Introduction Transport into and out of your cells is important because without cellular transport, the cell could not move material into or out of the cell. This would lead to its inability to survive because cells must take in oxygen and food, and expel waste material that could inhibit the cell from performing in the body. A type of cell transport in particular is passive transport.Passive transport is a kind oftransportby whichionsormoleculesmove along aconcentration gradient, which means movementfrom an area ofhigherconcentrationto an area oflowerconcentration. Passive transport does not require an siding of energy. There are 4 main types of passive transpor t simple diffusion, facilitated diffusion, filtration, and osmosis. In this lab, we are particularly looking at osmosis and how it affects plant and animal cells.Osmosis is the diffusion of water molecules across a selectively permeable membrane. In part A of this lab, we submerged the eggs in vinegar overnight, followed by peeling the softened shells from the eggs carefully era trying not to break the membrane the next day. We then recorded any observations we made on the eggs including weight and appearance. Next, we recorded any observations we could make on the carrots and potatoes before we put them into their solution.We then placed the eggs(animal cells), carrots, and potatoes(plant cells) into their solutions and let them stand in their hypotonic and hypertonic solutions overnight followed by observing any changes in weight and appearance the next day. In Part B, we observed an onion cell exposed to distilled water under a microscope, and another onion cell exposed to a sat urated salt solution, and drew the two different cells comparing the differences between their exposure to the hypotonic(water) and hypertonic(salt water, corn syrup) solutions. Part APurpose To observe the effects of hypotonic and hypertonic solutions on plant and animal cells. Hypothesis I predict that the hypotonic solution will cause the plant and animal cells to enlarge due to the difference in concentration on the inside of the cells, and the hypertonic solutions will cause the cells to shrink due to absorption of water molecules in the cells. Materials * 400mL beakers (2) * Tap Water * Eggs (2) * Vinegar * Salt water * Carrots (2) * Corn syrup * Potato strips (2) Procedure Day 1 1. Submerge the eggs in vinegar in a beaker. May have to weigh them down) 2. Leave them undisturbed overnight. Day 2 3. Peel the softened shells from the egg carefully so as not to break the 3membrane. 4. Record any pertinent observations on the egg, carrot and potato strip. 5. Place one egg and carro t in a beaker of water. 6. Place potato strip in a test tube. 7. Place the carrot in a beaker of saturated salt water and use corn syrup for the other egg. 8. Place the second potato strip in a test tube of saturated salt water. 9. Label the beakers and test tube and place in the cupboard overnight.Day 3 10. Record any pertinent observations. Observations Plant and Animal Cells in Hypertonic Solution Solution Appearance/texture Before Weight before(g) Appearance/texture After Weight After(g) Egg 1 Corn Syrup -translucent-yellowish colour-soft 77. 53 -opaque-white/yellow colour-feels firm 51. 17 Carrot 1 Salt solution -orange-sturdy 2. 59 -orange-sturdier -appears slightly smaller 2. 36 Potato 1 Salt solution -yellowish colour-sturdy 2. 92 -same colour-sturdier 2. 85 Plant and Animal Cells in hypotonic Solution(Distilled Water) Weight Before(g) Appearance/Texture After Weight After (g) Egg 2 71. 82 -opaque-orange colour 84. 10 Carrot 2 2. 29 -orange colour-not as sturdy 2. 52 Potato 2 2. 49 -yellowish colour-not as sturdy 2. 98 Part B Purpose To observe changes in plant cells when they are placed in a hypotonic(distilled water) solution and a hypertonic(saturated salt water) solution. Hypothesis I predict that the hypertonic solution will cause the water to leave the cell, shrinking it due to dehydration from the salt.Materials * Microscope * Microscope slide * Coverslip * Tap water * Saturated salt water * Onion Procedure 1. Prepare a wet mount using distilled water of a thin section of onion skin on a microscope slide. 2. View to draw one to two cells. Use magnification which shows the best detail. 3. Prepare another wet mount using the saturated salt solution instead of distilled water. 4. View and draw one to two cells. Use the same magnification you used for the first drawing. Observations Refer to onion cell drawings. AnalysisMy predictions were both correct in part A and in part B when I stated that the cells would shrink in the h ypertonic solution and enlarge in the hypotonic solution. The reason this occurred was because when a cell is immersed into a hypertonic solution, the tendency is for water to flow out of the cell in order to balance the concentration of the solute. When a cell is immersed in a hypotonic solution, it has a tendency to balance concentrations by water flowing into the cell, causing it to enlarge because of the lower concentration of solutes in its surroundings.None of the cells observed had the same weight when removed from their solution meaning they were not isotonic. Had they been isotonic, the solution would have no effect on the cells, therefore when placed in an isotonic solution the cells would tend to neither gain nor lose water. When observing the effects of hypotonic and hypertonic solutions on plant and animal cells, I came to the conclusion that the plant cells did not change much in weight. This is due to their cell walls which make it tougher for the cell to expand.The p ressure exerted by water inside the cell against the cell wall is known as turgor pressure. In the drawing of the onion cell exposed to a hypertonic solution, the cell membrane appeared to be shriveled up inside the cell wall due to its turgidity. Evaluation In part A, I did not experience any errors with the cells themselves, but potential sources of error could have been inaccurate measurements before and after placing the cells in their solution. Other potential sources of error could have occurred while peeling the shell off the egg, causing the membrane to break.In part B, I added too much distilled water to the slide making it difficult to get a good view of the onion cell exposed to the hypotonic solution. When performing a similar experiment in the future, I will be sure to add less drops with the eyedropper to the slide, enabling a clearer view of the cells I am observing. Application Questions 1. If the cells of a fresh water plant are placed in salt water, they will shriv el up due to the concentration difference between the salt water and the contents of the cell (cytoplasm). The water would escape the cell through osmosis causing it to dehydrate resulting in the shrinkage of the plant cell. . The fish that is used to salt water has high solutes and the freshwater is deficient of solutes, so when you place asaltwater fishin freshwater, the freshwater will move into the cells, causing them to swell and burst. 3. Grocery stores frequently spray the vegetables because the fresh water enters the plant cells through osmosis and makes them more(prenominal) turgid resulting in longer lasting freshness. 4. The use of road salt in the winter kills the plants alongside the road because the salt causes the cell to enter a hypertonic state. This causes the cytoplasm to move away from cell wall eventually making the plant shrivel up and die.Osmosis LabName ____Justine Basilone___ osmotic Regulation Virtual Laboratory biota Go to the following virtual laborator y http//www. glencoe. com/sites/common_assets/science/virtual_labs/LS03/LS03. html Answer your questions in this word document, save the file and make up through Edmodo. Your answers should be at to the lowest degree 2 complete sentences if not more Part I Observations & Data take on the Introductory information and the Procedure situated in the window to the left of the try out and then perform the laboratory. For your convenience, the data table has been inserted below fill this one in (the website tends to delete data) Molecules Name Red fund Cell Red Blood Cell Net Water impetus In / Out Appearance of Cell hypotonic Solution Blown up and fat Moves in to red blood cell Isotonic Solution Normal, did not appear different Moves in and out of red blood cell Hypertonic Solution Shriveled up Moves out of red blood cell ditchmoss genus ditchmoss Net Water Movement In / Out Appearance of Cell Hypotonic Solution Gets bigger Moves in to Elodea Isotonic S olution Appears the same. Moves in and out of Elodea (stays the same) Hypertonic Solution Moves out of Elodea The inside shrinks and gets smaller paramecium paramecium Net Water Movement In / Out Appearance of Cell Hypotonic Solution paramecium blows up and gets bigger. Moves in to Paramecium Isotonic Solution Seems to stay the same. Moves in and out of Paramecium Hypertonic Solution Shriveled up and makes the cell appear smaller. Moves out of Paramecium Part II Questions & Conclusions 1) Did water move into the cells or out of the cells while they were surrounded by hypotonic solution?Water moved into all of the cells while they were surrounded by hypotonic solution. 2) In which counselor did the water move through the cell membrane when the cells were surrounded by the hypertonic solution? The direction that the water appeared to be moving through the cell membrane was both ways, in and out. 3) Compare and pipeline what happens to an animal cell, a pla nt cell, and a Paramecium cell in a hypotonic, and isotonic, and a hypertonic solution. (Does the cell wall make a difference? ) What seemed to amazement me most is that no matter what cell was used each solution had the same effect of the cells, no matter what kind it was. So no, the cell wall does not appear to make a difference. ) Could Elodea or Paramecium from a freshwater lake be expected to survive if transplanted into the marine? Explain. Since they both react the same to different solutions I do believe that it is cipherable for both cells to be transplanted in the naval and survive. 5) If you were to grill a steak, would it be correct to put salt on it forrader or AFTER you cooked it? Explain wherefore, utilise THE TERMS OF OSMOSIS from this activity. While grilling a steak it is better to put salt on it before you grill it so while it is grilling osmosis will occur and it will retain the salt. So instead of it hardly sitting on top of the steak it will be on the inside. Giving it more flavor of the salt while feeding it. ) Why does salad become pulpy and limp when the ski binding has been on it for a while? Explain why, in terms of osmosis. The reason why salad becomes soggy and wilted when dressing has been on it for a while is because as it is sitting there osmosis takes place and the salad begins to take in the moisture of the salad dressing, leaving it soggy and wilted. 7) An effective way to kill weeds is to pour salt water on the ground around the plants. Explain why the weeds die, using the principles discovered in this virtual lab. I believe that the weeds would die due to the fact that as the weeds take in what they think is just water only to afterwards dry out by pickings in the salt along with it using osmosis.